Review Questions

1. A 27-year-old homeless mar) comes to the clinic because of a 3-week history of a fever, weight loss, night sweats, shortness of breath, and a cough with blood-tinged sputum. He says that a few of his acquaintances have similar symptoms. His temperature is 38.2°C (100.8°F), blood pressure is 120/80 mm Hg, pulse is 70/min, and respirations are 20/min. Crackles are heard over the upper lobes on auscultation. A chest x-ray shows upper lobe infiltrates and a 1-cm cavity in the left upper lobe. Three sputum samples are taken and sent to pathology for microscopic evaluation. Which of the following is the most appropriate histochemical stain to use for these specimens?

A. Acid-fast

B. Congo red

C. Periodic acid-Schiff

D. Prussian blue

E. Trichiome

2. A 48-year-old man comes to the physician because of an "odd discoloration" of his skin. He recently began to notice that he looks suntanned, even though it is the middle of the winter. A full work-up for this abnormal skin pigmentation shows diabetes mellitus and increased levels of serum ferritin and transferrin saturation. He is admitted to the hospital for a liver biopsy. The biopsy is sent to pathology for microscopic evaluation. In addition to hematoxylin and eosin (H&E), which of the following is the most appropriate histochemical stain to use for this specimen?

A

Acid-fast

B.

Congo red

C.

Gram stain

D.

Periodic acid-Schiff

E.

Prussian blue

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