FIGURE 10.7 Schematic representation of the fractionation procedure used to purify acidocalcisomes of T. gondii tachyzoites. The 15 000 x g pellet is applied in the middle layer of a 10-30 percent iodixanol stepgradient. Insets on the right are X-ray microanalysis of an isolated acidocalcisome showing the presence of carbon, oxygen, magnesium, phosphorus, and calcium, and a direct transmission electron micrograph (TEM) of the fraction showing the electron-dense acidocalcisomes. Bar = 1 pm. Reproduced with permission from Rodrigues et al. (2002), J. Biol. Chem. 277, 48 650-48 656.

from the ghosts present in the intermediate fractions, and a better recovery of the pellet fraction at the base of the gradient (Rodrigues et al., 2002).

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