Future Uses of Transgenic Fish

Fish are good candidates for transgenic studies. It now seems clear that our knowledge of fish genes and fish gene regulation will rapidly advance with the increased use of fish gene libraries and trans- genie induction with sequences derived from them. It also seems probable that, in the near future, transgenic fish with improved disease resistance, delayed sexual maturity, or increased growth rate will make a significant and valuable contribution to commercial aquaculture. Rainbow Trout These...

Injection of Zebra Fish Eggs

1 Fill the microinjection pipet with DNA solution see Section 2. . Place the pipet tip into about 50 pL of DNA solution on wax paper, and suck up into the pipet using the suction of a 6-mL syringe. 2. Inject the DNA using a continuously flowing pipet maintained with air pressure by an electrically driven automatic microinjector Fig. 3 , which maintains the pressure while injection is conducted. Precalibrate the amount injected by counting the number of seconds that the needle stays in the egg...