• Voided bladder 1 (VB1)—first 5-10 mL of voided urine and represents urethral flora
• Voided bladder 2 (VB2)—mid-stream urine and best correlates with bladder urine
• Voided bladder 3 (VB3)—initial 2-3 mL of urine, containing expressed prostatic secretions (EPS), collected following prostate massage
In patients with a suspected sexually transmitted infection (STI), urethritis, or urethral discharge, a VB1 and VB3 specimen are sent separately for culture.
• A bacterial count greater than 10 times in VB3 compared to VB1 or 2 is diagnostic of prostatitis
• A leucocyte count >10 per high-power field in VB3 compared to VB1 or 2 is diagnostic of prostatitis
• An EPS or VB3 pH of >8 is suggestive of prostatitis
• A higher bacterial count in VB1 compared to VB3 is diagnostic of urethritis
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