Stimulation Of Proliferation Of Hscs And Tissue Formation Of The Liver By A Longacting Vitamin C Derivative

A long-acting vitamin C derivative, l-ascorbic acid 2-phosphate (Asc 2-P), was found to stimulate cell proliferation, collagen accumulation, and tissue formation (Hata and Senoo, 1989; Kurata et al., 1993). On the basis of this discovery, Asc 2-P was added to the medium in which HSCs were cultured

FIGURE 13. Retraction of cellular processes by colchicine treatment. Rat HSCs cultured overnight on type I collagen gel were monitored for 1 h after the addition of 1 -mM colchicine. Pictures show phase-contrast video-enhanced micrographs 12 s (A), 15 min (B), 30 min (C), 45 min (D), and 60 min (E) after the addition of colchicine. Black arrowheads indicate the front of cellular process of the cell marked with white arrowheads. Scale bars indicate 100 mm.

FIGURE 13. Retraction of cellular processes by colchicine treatment. Rat HSCs cultured overnight on type I collagen gel were monitored for 1 h after the addition of 1 -mM colchicine. Pictures show phase-contrast video-enhanced micrographs 12 s (A), 15 min (B), 30 min (C), 45 min (D), and 60 min (E) after the addition of colchicine. Black arrowheads indicate the front of cellular process of the cell marked with white arrowheads. Scale bars indicate 100 mm.

(Senoo and Hata, 1994a). The cells in the medium supplemented with Asc 2-P stretched better than the cells in the control medium. Asc 2-P stimulated cell proliferation and collagen synthesis of the HSCs, and formation of the liver tissue-like structure in coculture of PCs and fibroblasts (Senoo et al., 1989).

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