Luciferase Assay

1 Homogenize tissue in 100 mM potassium phosphate, pH 7.8, and 1 mM DTT. Then prepare tissue extracts as for CAT, but do not heat to 65°C prior to assay (Notes 3 and 4).

2. Set up the incubation mixture as follows:

0.10 mL tissue extract 0.36 mL incubation buffer.

3. To initiate the reaction, inject 0.2 mL of luciferin solution into each sample using an automatic injection system. Measure integrated light output for 5 s after injection of luciferin using the free program mode of the luminometer.

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