Materials

1. M2 medium (see Chapter 16).

2. 70% Ethanol in squeeze bottle.

3. 2.5% Avertin (see Chapter 14) (or alternative anesthetic).

4. Light paraffin oil (Fluka [Buchs, Switzerland] or other; see Chapter 17).

5. 35-mm Sterile tissue-culture dishes

6. Hard glass capillaries, 1.5 mm od (BDH, Ltd., Poole, UK).

7. Mouth pieces, which also double as pipet holders (Arnold R. Horwell Ltd., West Hampstead, London, UK).

8. Pipet holders.

9. Flexible connecting tubing for pipet system.

Air cx

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