Notes

1 Excess bleeding can be prevented by a prior application of epinephrine (Sigma [St. Louis, MO], 20-50 pL of 0.1% solution diluted in 0.01M HC1) made through the bursa at the top of the oviduct using a fine needle (30 g) and 1-mL disposable syringe.

2 Although laboratory rodents can survive operations with minimal precautions against infection, it has been reported that treatment with antibiotic will increase oviduct-transfer efficiency, presumably by preventing minor infections of the oviduct. For rats, at least, a single postoperative injection of Binatol (50 mg, ip), an ampicillin-based product from Bayer, has been shown to be effective (Mullins, J. J., personal communication).

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