- Acknowledgments
- Additional Tests
- Aluminum Hydroxide
- Aluminum Phosphate
- Alum Precipitated Vaccines
- Assay of Copolymer Solution to Determine Dosing
- Assessment of Immunogenicity in Mice
- Assessment of TyVLP Yield and Purity
- Biology and Molecular Characterization of IL2
- By Covalent Coupling of the Antigen see Notes 3 4 and
- Chromium Release Assay - 2
- Cochleate Delivery Vehicles
- Conclusions
- Contributors
- Details of SAF Manufacture
- Determination of Antigen Loading Level in the Microparticles
- Determination of Microparticle Size
- DNA Cochleates
- Nase Digestion of DNA
- Dosing and Applications
- Emulsion Theory
- Entrapment by Large Particles Viruses or Bacteria Into Giant Liposomes
- Entrapment of Mumps Plasmid DNA into Virosomes see Note
- Estimation of Antigen Integrity
- ET ratio
- For Further Vaccine Formulation
- General Elisa Protocol
- Growth of Yeast to Induce TyVLP Production
- Hemolysis Test
- Humana Press Totowa New Jersey
- I
- Immunization of Mice
- Impediments to Rational Adjuvant Development
- In Process Assays for MF59C1
- In Vitro Release of Antigens from the Microparticles
- Iscomatrix Technology
- Karin Lovgren Bengtsson and Bror Morein 1 Introduction
- Limulus Lysate Assay
- Manipulation of Nucleic Acids Prior to Culture
- Manufacturing Considerations
- Materials - 2 3 4 5
- Materials see Subheading 211 6 7
- Measurement of Particle Size
- Measurement of Protein in the Presence of Copolymer to Determine Antigen Dose
- Methods
- Notes - 2 3 4 5 6 7 8 9 10 11 12
- Outline of Formulation
- Particle Size Determination
- Pelleting and Resuspension
- Plasmids and Yeast Strain
- Polymerization
- Preparation of Copolymer Solution
- Preparation of Formulations
- Preparation of ISCOMMatrix
- Preparation of ISCOMs Antigen Lipidation Techniques
- Preparation of Mouse Spleens for Bulk Culture Stimulation
- Preparation of MPL Oilin Water Emulsion
- Preparation of MPLTEoA Aqueous Dispersion
- Preparation of Plasmids
- Preparation of Primary Splenocyte Cultures
- Preparation of Stock MPL Oilin Water Emulsion Formulation
- Preparation of Stock MPLTEoA Aqueous Dispersion Formulation
- Preparation of the Emulsion - 2
- Principles of Assays for Immunostimulatory DNA
- Principles of
- Production of a Commercialized IRIVHepatitis A Vaccine see Note
- Purification of TyVLPs
- Pyrogenicity Tests
- Quantitation of Aluminum Adjuvants
- Quantitation of Cochleate Associated DNA
- Quantitation of Cochleate Associated Proteins
- References - 2
- Release Assays for MF59C1
- SAF Components and Chemistry
- Scale Up of Purification
- Stability Assays for MF59C1
- Stimulation of Splenocytes
- Stimulation of Splenocytes by DNA
- TCI on the Back see Note
- TCI on the Ear see Note
- The Mechanism of Action of Mineral Oil Adjuvants
- The Trypan Blue Test
- Trapping Methods
- Vaccine Adjuvant
- Yeast Cell Disruption
- Yeast Transformation - 2
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